ocr: T'he kinetics of enzyme catalyzed reactions are measured in a variety of piochemical and biological experiments. The equation of Michaelis and Menton provides a theoretical framework for determining two parameters toc characterize the kinetics of an enzyme catalyzed reaction, Vmax, - - the maximum velocity of the reaction, and Am ' the Michaelis-Menton constant. Km is equal to the substrate concentration atwhich the initial reaction velocity is half of the maximum velocity (ref. 1). la Vmax l Km lo 1 pa.hhl.4"